CST, 12774S, S-Tag (D2K2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, S-Tag (D2K2V) Rabbit Monoclonal Antibody (CST #12774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 80110 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity S-Tag (D2K2V) Rabbit Monoclonal Antibody recognizes transfected levels of recombinant protein containing an S-Tag. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic S-Tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. S-Tag is an epitope tag composed of a 15 residue peptide that, together with S-protein, comprises pancreatic ribonuclease S (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG