CST, 12929S, TMEM49/VMP1 (D1Y3E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TMEM49. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM49/VMP1 (D1Y3E) Rabbit Monoclonal Antibody (CST #12929) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TMEM49/VMP1 (D1Y3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMEM49/VMP1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TMEM49/VMP1 protein. Background Vacuole membrane protein 1 (VMP1, TMEM49) is a transmembrane protein localized to intracellular vacuoles that was originally described as a protein promoting vacuole formation in acinar cells associated with acute pancreatitis (1). Over-expression of VMP1 promotes vacuole formation and subsequent cell death (1). Additional research studies demonstrated that VMP1 expression might be induced by starvation or the mTOR inhibitor rapamycin, which triggers autophagy (2). VMP1 is targeted along with LC3 to autophagosome membranes (2). Knockdown of VMP1 can inhibit autophagosome formation (2). VMP1 interacts with beclin-1, a key autophagy protein that activates the class III PI3 kinase Vps34 (3). VMP1 functions in the degradation and clearance of zymogen-containing vacuoles during experimentally induced pancreatitis (4). During vacuole degradation and clearance, VMP1 interacts with the ubiquitin protease USP9X, suggesting a possible functional link between the molecular machinery of autophagy and the ubiquitin pathway. Orthologs of VMP1 from (known as EPG-3), (known as TANGO-5), and , have been shown to play a role in membrane trafficking, organelle organization, and autophagy (5-7). Alternate Names DKFZp566I133; ectopic P-granules autophagy protein 3 homolog; EPG3; TANGO5; TDC1; TMEM49; TMM49; Transmembrane protein 49; transport and golgi organization 5 homolog; Vacuole membrane protein 1; VMP1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG