CST, 12963S, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying cytochrome C. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody (CST #12963) is ready to ship. Product Usage Information Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:300 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cytochrome c (6H2.B4) Mouse Monoclonal Antibody recognizes endogenous levels of total cytochrome c protein by immunofluorescence. This antibody is not recommended for western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant rat cytochrome c protein. Background Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). Alternate Names CYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Mouse IgG1