CST, 12968S, RAIG1 (D4S7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAIG1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, RAIG1 (D4S7D) Rabbit Monoclonal Antibody (CST #12968) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73117 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RAIG1 (D4S7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total RAIG1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val295 of human RAIG1 protein. Background Retinoic Acid-Induced Gene 1 (RAIG1), also called RAI3 and GPRC5A, belongs to the family of G protein-coupled receptors (GPCRs). GPCRs are the largest and most abundant receptor family in mammals, composed of more than one thousand members (1). Retinoic acids (RA) are a group of pleiotropic signaling molecules that regulate a broad range of physiologic and developmental processes. It is thought that RAIG1 may be a downstream target of RA signaling (2). RAIG1 is expressed in lung tissue and in several lung cancer cell lines, and encodes a seven-transmembrane glycoprotein presumed to be a lung tumor suppressor gene (3,4). Alternate Names G protein-coupled receptor class C group 5 member A; G protein-coupled receptor, family C, group 5, member A; G-protein coupled receptor family C group 5 member A; GPCR5A; GPRC5A; Orphan G-protein-coupling receptor PEIG-1; PEIG-1; Phorbol ester induced gene 1; phorbol ester induced protein-1; RAI3; RAIG-1; RAIG1; retinoic acid induced 3; retinoic acid responsive; Retinoic acid-induced gene 1 protein; Retinoic acid-induced protein 3; TIG1; TPA induced gene 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit