CST, 12975T, Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MRLC2V mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit Monoclonal Antibody (CST #12975) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit Monoclonal Antibody recognizes endogenous levels of total myosin light chain 2v (cardiac isoform) protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse myosin light chain 2v (cardiac isoform) protein. Background Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms with varying tissue distribution. Smooth muscle MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca /calmodulin-dependent manner (1). These phosphorylation events are correlated with myosin ATPase activity and smooth muscle contraction (2). Striated muscle contraction is regulated by the troponin-tropomyosin complex in thin actin filaments and by binding of Ca to troponin C (3). Two types of myosin light chain are expressed in the heart, with myosin light chain 2v (MYL2, MLC-2v) expression restricted to the ventricles and myosin light chain 2a (MYL7, MLC-2a) found specific to the atria. Mutations in the corresponding gene are found in patients diagnosed with a form of hypertrophic cardiomyopathy characterized by thickening of the mid-left ventricle. Alternate Names Gm32672; Mlc; MLC-; MLC-2; MLC-2s/v; MLC-2v; Mlc2v; MLRV; MRLC2V; Myl; Myl2; Mylpc; Myosin light chain 2, slow skeletal/ventricular muscle isoform; myosin light chain 2v; myosin light chain, phosphorylatable, cardiac ventricles; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin, light polypeptide 2, regulatory, cardiac, slow; OTTMUSP00000029571; OTTMUSP00000029619; ventricular myosin regulatory light chain Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG