CST, 12979S, Filamin B (D7E4W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Filamin B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Filamin B (D7E4W) Rabbit Monoclonal Antibody (CST #12979) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Filamin B (D7E4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total filamin B protein. Based on the amino acid sequence this antibody is not expected to cross-react with filamin A or filamin C. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg691 of human filamin B protein. Background Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (α-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as β-integrins, Rho/Rac/Cdc42, PKC and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4). Alternate Names ABP-278; ABP-280; ABP-280 homolog; actin binding protein 278; Actin-binding-like protein; AOI; Beta-filamin; DKFZp686A1668; DKFZp686O033; Fh1; filamin 1 (actin-binding protein-280)-like; filamin B; filamin B, beta; Filamin homolog 1; Filamin-3; Filamin-B; FLN-B; FLN1L; FLN3; FLNB; Larsen syndrome 1 (autosomal dominant); LRS1; SCT; TABP; TAP; Thyroid autoantigen; Truncated ABP; Truncated actin-binding protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG