CST, 12988T, MSH6 (3E1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MSH6. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MSH6 (3E1) Mouse Monoclonal Antibody (CST #12988) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98142 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MSH6 (3E1) Mouse Monoclonal Antibody recognizes endogenous levels of total MSH6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human MSH6 protein expressed in . Background The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4). Alternate Names DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS homolog 6; mutS homolog 6 (E. coli); MutS protein homolog 6; MutS-alpha 160 kDa subunit; p160; sperm-associated protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Mouse IgG2a