CST, 13013S, Spry1 (D9V6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Spry1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Spry1 (D9V6P) Rabbit Monoclonal Antibody (CST #13013) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Spry1 (D9V6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total Spry1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg70 of human Spry1 protein. Background Spry1 is a member of the Sprouty (Spry) family proteins that was initially identified in as an inhibitor of the FGF signaling pathway (1). There are four human Spry proteins (Spry1-4), encoded by different genes, and they all share a highly conserved carboxy-terminal cystine-rich Spry domain that is known to be essential for their receptor tyrosine kinase inhibitory function stimulated by various growth factors (1-3). Spry1 and other Spry proteins play a key role in embryonic development, tissue and organ formation, as well as growth in almost all living organisms (1-4). Spry proteins are considered tumor suppressors due to their inhibitory function in a variety of growth factor signaling pathways (2,3). Spry1 anchors itself to the membrane by palmitoylation and can translocate from the cytosol to the membrane by binding to caveolin-1 (5,6). Regulation of Spry1 protein function is thought to occur at various levels. Spry1 regulation includes transcriptional regulation by growth factors and kinases (1,4,7), post-transcriptional regulation by microRNA-21 (8), post-translational modifications including phosphorylation, dephosphorylation, ubiquitination and proteasomal degradation, and regulation by its interacting protein partners (2,3). Alternate Names hSPRY1; Protein sprouty homolog 1; sprouty homolog 1, antagonist of FGF signaling; sprouty homolog 1, antagonist of FGF signaling (Drosophila); sprouty RTK signaling antagonist 1; sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling); Spry-1; SPRY1; SPY1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG