CST, 13014S, IRF-7 (D2A1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRF-7. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, IRF-7 (D2A1J) Rabbit Monoclonal Antibody (CST #13014) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRF-7 (D2A1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRF-7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro115 of human IRF-7 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). IRF-7 is regulated at multiple serine phosphorylation sites near the carboxyl terminus, similar to IRF-3, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11). Alternate Names IMD39; Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG