CST, 13031S, LKB1 (D60C5F10) Rabbit Monoclonal Antibody (IHC Formulated)

Monoclonal Antibody for studying LKB1. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LKB1 (D60C5F10) Rabbit Monoclonal Antibody (IHC Formulated) (CST #13031) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28193 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity LKB1 (D60C5F10) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total LKB1 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LKB1 protein. Background LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7). Alternate Names hLKB1; Liver kinase B1; LKB1; PAR-4; PAR4; PJS; polarization-related protein LKB1; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Serine/threonine-protein kinase STK11; STK11 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG