CST, 13060S, VAMP8 Antibody

Polyclonal Antibody for studying VAMP8. Validated for Western Blotting. Highly specific and rigorously validated in-house, VAMP8 Antibody (CST #13060) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VAMP8 Antibody recognizes endogenous levels of total VAMP8 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val31 of human VAMP8 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle associated membrane protein 8 (VAMP8), also known as endobrevin, is a v-SNARE originally found preferentially localized to early endosomes (3). VAMP8 knockout mice did not show abnormal endosomal vesicular trafficking, perhaps having a redundant role with other VAMP family members (4). Instead, research studies have shown that VAMP8 is widely expressed in exocrine tissues and has a critical role in the exocytosis pathways of a variety of cells (4-9). In addition, lysosome localized VAMP8 has been shown to play a role in autophagosome/lysosome fusion during antimicrobial (xenophagy) and canonical starvation induced autophagy (5). Alternate Names EDB; Endobrevin; VAMP-8; VAMP8; vesicle associated membrane protein 8; Vesicle-associated membrane protein 8; vesicle-associated membrane protein 8 (endobrevin) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 15 SOURCE: Rabbit