CST, 13084S, Wee1 (D10D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Wee1. Validated for WB,WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Wee1 (D10D2) Rabbit Monoclonal Antibody (CST #13084) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53692 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Wee1 (D10D2) Rabbit Monoclonal Antibody recognizes endogenous levels of total Wee1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala54 of human Wee1 protein. Background Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7). Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by β-TrCP-mediated ubiquitination and degradation (1,9,10). Alternate Names DKFZp686I18166; FLJ16446; WEE1; WEE1 G2 checkpoint kinase; WEE1 homolog; Wee1-like protein kinase; WEE1+ homolog; WEE1A; Wee1A kinase; WEE1hu Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG