CST, 13123T, PFKFB3 (D7H4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PFKFB3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PFKFB3 (D7H4Q) Rabbit Monoclonal Antibody (CST #13123) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PFKFB3 (D7H4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total PFKFB3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu456 of human PFKFB3 protein. Background The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). One of them, PFKFB3/iPFK-2, was shown to be inducible by hypoxia leading to increased glycolysis under hypoxic conditions (2). Research studies have shown that PFKFB3/iPFK-2 is also highly expressed in some types of human cancer (3). Alternate Names 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; F263; FLJ37326; Fructose-2,6-bisphosphatase; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY-REN-56 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG