CST, 13136S, TRF2 (D1Y5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRF2 (D1Y5D) Rabbit Monoclonal Antibody (CST #13136) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRF2 (D1Y5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human TRF2 protein. Background Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins TRF1 and TRF2 bind to double-stranded telomeres to allow formation of T-loops (2). A large number of proteins involved in the DNA damage response are found physically associated with TRF2 within telomeres (3). Interestingly, TRF2 can transiently localize to DNA damage-induced DSBs, but overexpression of TRF2 prevents ATM-dependent signaling (4). Phosphorylation of TRF2 at Ser323 has been reported , but no upstream kinase or role has been established for this phosphorylation site (5). Alternate Names Telomeric DNA-binding protein; telomeric repeat binding factor 2; telomeric repeat binding protein 2; Telomeric repeat-binding factor 2; TERF2; TRBF2; TRF2; TTAGGG repeat-binding factor 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG