CST, 13158S, NLRP3 (D2P5E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLRP3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP3 (D2P5E) Rabbit Monoclonal Antibody (CST #13158) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP3 (D2P5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro92 of human NLRP3 protein. Background The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). NLRP3 is an inflammasome-forming NLR that activates caspase-1 leading to maturation of IL-1β and IL-18 (8). The NLRP3 inflammasome is assembled in response to a wide variety of microbial and endogenous stimuli, and therefore it is unlikely the NLRP3 directly interacts with its activators (8). NLRP3 is expressed at highest levels in dendritic cells, monocytes, and macrophages (9). Alternate Names AGTAVPRL; AII; AII/AVP; AII/AVP receptor-like; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; FLJ95925; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family pyrin domain containing 3; NLR family, pyrin domain containing 3; NLRP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85,110 Source/Isotype: Rabbit IgG