CST, 13166T, Integrin beta3 (D7X3P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Beta3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta3 (D7X3P) Rabbit Monoclonal Antibody (CST #13166) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:125 - 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 29462 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Integrin beta3 (D7X3P) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin β3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile114 of human integrin β3 protein. Background Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). αIIβ3 and αVβ3 are the two β3 containing integrins which are prominently expressed in hematopoietic cells and angiogenic endothelial cells and perform adhesive functions in hemostasis, wound healing and angiogenesis (1,4). Tyr773 and Tyr785 (usually referred to as Tyr747 and Tyr759 based on the chicken sequence) are phosphorylated upon ligand binding (5). Phosphorylation of these tyrosine residues is required for certain ligand-induced signaling (6). Thr779 (corresponding to Thr753 of the chicken sequence) of integrin β3 in the platelet specific αIIβ3 is phosphorylated by PKD and/or Akt, which may modulate integrin association with other signaling molecules (7). Alternate Names antigen CD61; BDPLT16; BDPLT2; CD61; GP3A; GPIIIa; GT; integrin beta 3; integrin beta chain, beta 3; Integrin beta-3; integrin subunit beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITB3; ITGB3; platelet glycoprotein IIIa; Platelet membrane glycoprotein IIIa Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG