CST, 13171S, RBBP5 (D3I6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBBP5. Validated for WB,IP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, RBBP5 (D3I6P) Rabbit Monoclonal Antibody (CST #13171) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity RBBP5 (D3I6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBBP5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val408 of human RBBP5 protein. Background The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6). Alternate Names RB binding protein 5, histone lysine methyltransferase complex subunit; RBBP-5; RBBP5; RBQ3; retinoblastoma binding protein 5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1; SWD1, Set1c WD40 repeat protein, homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG