CST, 13175T, Phospho-PRAS40 (Thr246) (D4D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRAS40 (Thr246) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PRAS40 (Thr246) (D4D2) Rabbit Monoclonal Antibody (CST #13175) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 32263 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-PRAS40 (Thr246) (D4D2) Rabbit Monoclonal Antibody recognizes endogenous levels of PRAS40 protein only when phosphorylated at Thr246. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40 protein. Background Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). Alternate Names 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1S1; AKTS1; Lobe; PRAS40; Proline-rich AKT1 substrate 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit