CST, 13185S, AMPA Receptor 1 (GluA1) (D4N9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GluR1. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, AMPA Receptor 1 (GluA1) (D4N9V) Rabbit Monoclonal Antibody (CST #13185) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 39325 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity AMPA Receptor 1 (GluA1) (D4N9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total AMPA Receptor 1 (GluA1) protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human AMPA Receptor 1 (GluA1) protein. Background AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluA 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluA 2-containing AMPARs, AMPARs that lack GluA 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer's, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). GluR 1 is necessary for expression of long-term potentiation (LTP) in the hippocampus and formation of short-term memory (3). Hippocampal GluR 1 is also involved in morphine-induced adaptive synaptic mechanisms (4). Alternate Names AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; glutamate ionotropic receptor AMPA type subunit 1; Glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GRIA1; HBGR1; MGC133252 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG