CST, 13192S, Golgin-97 (D8P2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Golgin97. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Golgin-97 (D8P2K) Rabbit Monoclonal Antibody (CST #13192) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Golgin-97 (D8P2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total golgin-97 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu663 of human golgin-97 protein. Background The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88, and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the TGN through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain TGN integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9). Alternate Names gap junction protein, alpha 4, 37kD; GOGA1; GOLGA1; golgi autoantigen, golgin subfamily a, 1; golgin A1; Golgin subfamily A member 1; Golgin-97; MGC33154 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG