CST, 13233T, DCP1B (D2P9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCP1B. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DCP1B (D2P9W) Rabbit Monoclonal Antibody (CST #13233) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DCP1B (D2P9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCP1B protein. This antibody may cross-react with DCP1A. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val202 of human DCP1B protein. Background mRNA decapping is an important process in the mRNA turnover (1). DCP1A and DCP2 were identified as two human decapping enzymes and homologs of the better-characterized enzymes. Both putative decapping enzymes interact with the regulator of nonsense transcripts 1 (UPF1) and may be recruited by UPF1 or related proteins to mRNA sequences that contain premature termination codons (1). Additional research studies demonstrate that DCP1A, DCP1B (the homolog of DCP1A) and DCP2 colocalize with decapping activation factors RCK/p54 and Lsm proteins in cytoplasmic loci (2). DCP1A, DCP1B and DCP2 are components of cytoplasmic processing (P) bodies, with hyper-phosphorylation of DCP1A during mitosis suggesting a possible mechanism of P-body regulation during the cell cycle (3,4). Alternate Names DCP1; DCP1 decapping enzyme homolog B; DCP1 decapping enzyme homolog B (S. cerevisiae); DCP1B; decapping enzyme hDcp1b; decapping mRNA 1B; hDcp1b; mRNA-decapping enzyme 1B Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG