CST, 13251T, GPNMB (E1Y7J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPNMB. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GPNMB (E1Y7J) Rabbit Monoclonal Antibody (CST #13251) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GPNMB (E1Y7J) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPNMB protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp247 of human GPNMB protein. Background Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein overexpressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes (1), regulating osteoblast and osteoclast differentiation and function (2), stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells (3), enhancing autophagosome fusion to lysosomes in tissue repair, and regulating degradation of cellular debris (4,5). While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11). The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13). Alternate Names glycoprotein (transmembrane) nmb; glycoprotein NMB; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; HGFIN; NMB; osteoactivin; PLCA3; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 120 Source/Isotype: Rabbit IgG