CST, 13299T, Semaphorin 3B (D5A2P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SEMA3B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Semaphorin 3B (D5A2P) Rabbit Monoclonal Antibody (CST #13299) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Semaphorin 3B (D5A2P) Rabbit Monoclonal Antibody recognizes endogenous level of total semaphorin 3B protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln717 of human semaphorin 3B protein. Background Semaphorins are a family of cell surface and secreted proteins initially recognized as axon guidance factors that control the development of the central nervous system (1). They are involved in cell migration, angiogenesis, and immune responses (2-6). Based on protein structure, there are eight classes of semaphorins. Class 3-7 semaphorins are expressed in vertebrates. Semaphorin 3 subfamily members are the only secreted semaphorins in vertebrates. There are seven semaphorin 3 proteins and their receptors include neuropilins and the type A/D family plexins (7-9). Semaphorin 3B functions as a tumor suppressor, as research studies have shown that it is deleted or inactivated in lung and breast cancer (10,11). Overexpression of semaphorin 3B inhibits tumor cell proliferation and causes apoptosis (12,13). Semaphorin 3B also inhibits angiogenesis (14). Semaphorin 3B loses its activity upon cleavage by furin-like pro-protein convertases (14). Alternate Names LUCA-1; SEM3B; SemA; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; Sema V; SEMA3B; SEMA5; SEMAA; semaphorin 3B; semaphorin A; Semaphorin-3B; Semaphorin-V; semaV Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG