CST, 13331T, CDK6 (D4S8S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cdk6. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK6 (D4S8S) Rabbit Monoclonal Antibody (CST #13331) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62382 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CDK6 (D4S8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK6 protein. This antibody does not cross-react with other CDK proteins. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK6 protein. Background The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4). Alternate Names CDK6; CDKN6; Cell division protein kinase 6; cyclin dependent kinase 6; Cyclin-dependent kinase 6; MCPH12; MGC59692; PLSTIRE; Serine/threonine-protein kinase PLSTIRE Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG