CST, 13355T, CD102/ICAM-2 (D7P2Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ICAM2. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD102/ICAM-2 (D7P2Q) Rabbit Monoclonal Antibody (CST #13355) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CD102/ICAM-2 (D7P2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD102 (ICAM-2) protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CD102 (ICAM-2) protein. Background Intercellular cell adhesion molecule-2 (CD102/ICAM-2) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. Like CD54/ICAM-1, CD102/ICAM-2 is a ligand that binds the leukocyte adhesion molecule LFA-1 (leukocyte function-associated antigen-1), which mediates intercellular interactions between immune cells and other cell types (1). Expression of CD102/ICAM-2 has been shown to affect angiogenesis (2), cellular radioresistance (3) and anti-tumor immune response (4). Along with CD54/ICAM-1, CD102/ICAM-2 mediates T cell crawling and diapedesis across the blood-brain barrier (5), as well as T cell migration across the bronchial epithelium (6). CD102/ICAM-2 interaction with the actin cytoskeleton through α-actinin has been shown to limit the mobility on neuroblastoma cells (7), and this effect is dependent on glycosylation of CD102/ICAM-2 (8). Alternate Names CD102; ICAM-2; ICAM2; Intercellular adhesion molecule 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45, 55 Source/Isotype: Rabbit IgG