CST, 13377T, TNF-R1 (D3I7K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNF-R1 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TNF-R1 (D3I7K) Rabbit Monoclonal Antibody (CST #13377) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TNF-R1 (D3I7K) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse TNF-R1 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TNF-R1 protein. Background TNF-α is an important cytokine produced by numerous cell types, including neutrophils, activated lymphocytes, macrophages, and NK cells. It plays a critical role in inflammatory responses and apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (9,10). Alternate Names CD120; CD120a; FPF; OTTMUSP00000028139; p55; p55-R; p60; Tnf; TNF receptor alpha chain; TNF-; TNF-a; TNF-alpha-R1; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNF-RI; TNFal; TNFalpha-R1; TNFAR; TNFR; Tnfr-1; Tnfr-2; TNFR-I; Tnfr1; TNFR60; TNFRI; TNFRp55; Tnfrsf1a; TNR1A; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1a; Tumor necrosis factor receptor type I Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG