CST, 13381S, Artemis (D7O8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Artemis. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Artemis (D7O8V) Rabbit Monoclonal Antibody (CST #13381) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Artemis (D7O8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total artemis protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg377 of human artemis protein. Background DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. Defects in both pathways have been associated with human disease, including cancer (1). Artemis is a ubiquitously expressed NHEJ factor that exhibits endonuclease activity. Artemis functions in DNA repair by promoting nonhomologous end joining (2), as well as in cell cycle checkpoint control through ATM/ATR signaling (3). NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes, and artemis is a key factor in this process (4,5). Mutations in the corresponding artemis gene ( ) are associated with a radiosensitive type of severe combined immunodeficiency (SCID) in humans (6,7). Alternate Names A-SCID; A-SCID protein; ARTEMIS; artemis protein; ASCID; DCLRE1C; DCLREC1C; DCR1C; DNA cross-link repair 1C; DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1C protein; FLJ11360; FLJ36438; hSNM1C; Protein A-SCID; Protein artemis; RS-SCID; SCIDA; severe combined immunodeficiency, type a (Athabascan); SNM1 homolog C; SNM1-like protein; SNM1C Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG