CST, 13389S, PGD Antibody

Polyclonal Antibody for studying PGD. Validated for Western Blotting. Highly specific and rigorously validated in-house, PGD Antibody (CST #13389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PGD Antibody recognizes endogenous levels of total PGD protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala300 of human PGD protein. Antibodies are purified by protein A and peptide affinity chromatography. Background 6-phosphogluconate dehydrogenase (PGD or 6PGD) catalyzes the conversion of 6-phosphogluconate and NADP to ribulose 5-phosphate and NADPH in the pentose phosphate pathway (1). Research studies show that knockdown of PGD results in the induction of senescence and inhibition of growth of lung cancer cells (2). Additional research suggests that PGD influences the migration of some cancer cells by regulating c-Met phosphorylation state (3). Furthermore, it was shown that the glycolytic enzyme PGAM1 substrate (3-phosphoglycerate) regulates the pentose phosphate pathway by deactivating PGD/6PGD (4). Alternate Names 6-phosphogluconate dehydrogenase; 6-phosphogluconate dehydrogenase, decarboxylating; 6PGD; PGD; PGDH; phosphogluconate dehydrogenase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit