CST, 13398S, KHSRP (E2E2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KHSRP. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, KHSRP (E2E2U) Rabbit Monoclonal Antibody (CST #13398) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KHSRP (E2E2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total KHSRP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val78 of human KHSRP protein. Background KHSRP, also known as KSRP, is a KH domain-containing AU-rich element (ARE) binding protein (1). It recruits degradation machinery and activates mRNA turnover (2). This protein was previously shown to function as a regulator for splicing (3). KHSRP associates with both the Drosha and Dicer multiprotein complexes (4), and controls the biogenesis of some microRNAs by binding to the terminal loops of these microRNA precursors (3). KHSRP is found in neural and non-neural cell types in both the nucleus and the cytoplasm (4). Alternate Names epididymis secretory sperm binding protein; Far upstream element-binding protein 2; FBP2; FUBP2; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein; KH-type splicing regulatory protein; KHSRP; KSRP; MGC99676; p75 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG