CST, 13419S, E-Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, E-Tag Antibody (CST #13419) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity E-Tag Antibody recognizes transfected levels of recombinant proteins containing an E-tag. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic E-tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. E-tag is an epitope tag composed of a 13 residue peptide. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1,2). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit