CST, 13422S, Choline Kinase alpha (D5X9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHOK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Choline Kinase alpha (D5X9W) Rabbit Monoclonal Antibody (CST #13422) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Choline Kinase alpha (D5X9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total choline kinase α protein. Based on the antigen sequence, this antibody is not expected to recognize choline kinase β. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human choline kinase α protein. Background Choline kinase (ChoK) catalyzes the phosphorylation of choline, a key step in the biosynthesis of the membrane phospholipid phosphatidylcholine. At least three ChoK isoforms exist in mammalian cells, α-1, α-2, and β. The two α isoforms are transcribed from the same gene as splice variants, while the β isoform resides on a separate gene (reviewed in 1). Research studies indicate that ChoKα levels affect signaling through MAPK and Akt pathways (2,3). Investigators have shown that ChoKα plays a role in proliferation and carcinogenesis and is highly expressed/activated in human cancers (4-7). Additional research studies suggest ChoKα may be a potential target for cancer therapy (8). Alternate Names CHETK-alpha; CHK; CHKA; Choline kinase alpha; CK; CKI; EK; Ethanolamine kinase Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG