CST, 13437T, EXPAND1/MUM1 (D1Z6Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MUM1/EXPAND1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, EXPAND1/MUM1 (D1Z6Y) Rabbit Monoclonal Antibody (CST #13437) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EXPAND1/MUM1 (D1Z6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total EXPAND1/MUM1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg692 of human EXPAND1/MUM1 protein. Background DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes. In response to DNA damage, EXPAND1/MUM1 accumulates at sites of DNA double strand breaks through direct interaction with DNA repair factor 53BP1 (1). Accumulation of EXPAND1/MUM1 at damaged DNA sites is thought to modify the structure of the chromatin and allow access to other DNA repair factors (2). 53BP1 activates the checkpoint kinase ATM and promotes DNA double strand break repair via nonhomologous end joining (NHEJ) repair pathway (3). Alternate Names EXPAND1; FLJ14868; FLJ22283; HSPC211; melanoma associated antigen (mutated) 1; melanoma ubiquitous mutated protein; MGC131891; MGC163315; MUM-1; MUM1; Mutated melanoma-associated antigen 1; Protein expandere; PWP3A; PWWP domain containing 3A, DNA repair factor; PWWP domain-containing DNA repair factor 3A; PWWP domain-containing protein MUM1; PWWP3A Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG