CST, 13439S, Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PCNA (Lys164) ubiquitinate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit Monoclonal Antibody (CST #13439) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 94435 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit Monoclonal Antibody recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein. Background Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation. PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5). Alternate Names ATLD2; Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 44 Source/Isotype: Rabbit IgG