CST, 13451S, DNMT3L (E1Y7Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DNMT3L mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DNMT3L (E1Y7Q) Rabbit Monoclonal Antibody (CST #13451) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DNMT3L (E1Y7Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNMT3L protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val385 of mouse DNMT3L protein. Background Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2). These methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4,5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7). Alternate Names D6Ertd14; D6Ertd14e; DNA (cytosine-5-)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; DNM3L; Dnmt3l; ecat; ecat7; MGC102308 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG