CST, 13460S, CHD1L (E1I8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHD1L. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHD1L (E1I8C) Rabbit Monoclonal Antibody (CST #13460) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHD1L (E1I8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHD1L protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly231 of human CHD1L protein. Background Chromodomain-helicase-DNA-binding domain 1 like (CHD1L) is a DNA helicase and member of the SNF2 subfamily of ATP dependent chromatin remodeling enzymes (1). Unlike other CHD chromatin remodeling proteins, CHD1L lacks a methylated histone binding chromodomain but does contain a macro domain that binds poly-ADP ribosylated (PARylated) targets (1,2). Following genotoxic stress, CHD1L interacts with PARylated PARP1 and is recruited to sites of DNA damage to facilitate DNA repair (3,4). The CHD1L protein is often over expressed in metastatic hepatocellular carcinoma (HCC) and the corresponding gene is located in a region that is frequently amplified in cases of HCC (5-7). Research studies indicate that CHD1L over expression may lead to over relaxation of chromatin and exposing the underlying DNA to genotoxic stress (1). CHD1L can regulate expression of genes that promote tumor cell proliferation, migration and metastasis, providing another mechanism where CHD1L may promote hepatocellular carcinoma progression and metastasis (6,8). Additional research studies suggest that over expression of CHD1L may be involved in the progression of bladder, colon and ovary cancer (9-11). Alternate Names ALC1; amplified in liver cancer 1; Amplified in liver cancer protein 1; CHD1L; CHDL; chromodomain helicase DNA binding protein 1 like; chromodomain helicase DNA binding protein 1-like; Chromodomain-helicase-DNA-binding protein 1-like; FLJ22530 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG