CST, 13465T, XPF (D3G8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ERCC4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, XPF (D3G8C) Rabbit Monoclonal Antibody (CST #13465) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity XPF (D3G8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total XPF (ERCC4) protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg670 of human XPF (ERCC4) protein. Background DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 4 (ERCC4, XPF) forms a complex with the ERCC1 excision repair protein to create an essential 5' endonuclease responsible for lesion excision (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Mutations in the corresponding gene cause specific forms of xeroderma pigmentosum, Cockayne syndrome, and Fanconi anemia, while altered XPF protein levels may be associated with disease progression and response to treatment in specific human cancers (5,6). Alternate Names DNA excision repair protein ERCC-4; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; ERCC excision repair 4, endonuclease catalytic subunit; ERCC11; ERCC4; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; FANCQ; RAD1; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F; XFEPS; XPF Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 104 Source/Isotype: Rabbit IgG