CST, 13490T, PACT (D9N6J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PACT. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PACT (D9N6J) Rabbit Monoclonal Antibody (CST #13490) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PACT (D9N6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PACT protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PACT protein. Background PACT (protein activator of protein kinase R) is a double stranded RNA binding protein and a cellular activator of PKR (protein kinase R), a kinase that mediates the antiviral and antiproliferative actions of interferon (1). Stress signals such as serum starvation and arsenite treatment induces PACT to heterodimerize with PKR, resulting in the phosphorylation of eIF-2α and inhibition of protein synthesis (1,2). PACT has also been shown to play a role in RNA-mediated gene silencing by stimulating the activity of Dicer, thereby affecting the efficiency of miRNA accumulation and siRNA gene silencing (3,4). More recently, PACT has been shown to interact with RIG-I, a sensor of viral nucleic acids, and stimulates RIG-I-induced type I interferon production (5). Researchers have found that mutations in PACT are associated with dystonia, a movement disorder where patients develop involuntary muscle contractions and postures (6-8). Alternate Names DYT16; HSD14; Interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PACT; PKR-associated protein X; PKR-associating protein X; PRKRA; protein activator of interferon induced protein kinase EIF2AK2; Protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA dependent activator; Protein kinase, interferon-inducible double stranded RNA-dependent activator; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; RAX Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG