CST, 13508S, VAMP2 (D6O1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VAMP2. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, VAMP2 (D6O1A) Rabbit Monoclonal Antibody (CST #13508) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Immunofluorescence (Frozen): 1:2000 Immunofluorescence (Immunocytochemistry): 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 50423 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity VAMP2 (D6O1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP2 protein. This antibody does not cross-react with VAMP1 protein and recognizes VAMP2 protein by IF-IC analysis in mouse and rat cells. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human VAMP2 protein. Background Vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin) is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium (2). In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release (2). In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils (3) and release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5). Alternate Names FLJ11460; NEDHAHM; SYB2; synaptobrevin 2; Synaptobrevin-2; VAMP-2; VAMP2; vesicle associated membrane protein 2; Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit