CST, 13552S, Prolactin Receptor (D4A9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Prolactin receptor. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Prolactin Receptor (D4A9) Rabbit Monoclonal Antibody (CST #13552) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Prolactin Receptor (D4A9) Rabbit Monoclonal Antibody recognizes endogenous levels of total prolactin receptor protein. This antibody detects both long (90 kDa) and short (65 kDa) isoforms of the prolactin receptor. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp518 of human prolactin receptor protein. Background Prolactin receptor (PRLR) is a single-pass transmembrane receptor that mediates the actions of prolactin, a peptide hormone secreted by the anterior pituitary. PRLR is a type 1 cytokine receptor that is best known for promoting lactation in mammals, but which is also implicated in osmoregulation, metabolism, and immune system function (1). Research studies suggest that PRLR activation may promote tumor growth (2). Prolactin signaling via PRLR can activate multiple signal transduction pathways in breast cancer cells, including the Jak/Stat, PI3K/Akt, and MAPK pathways, leading to both pro-proliferative and anti-apoptotic downstream effects (3,4). Nine isoforms of PRLR have been identified, with the canonical (long) isoform primarily responsible for the pro-oncogenic effects of PRLR in some cancer cell lines (3). Much less is known about the functions of the other prolactin receptor isoforms. Defining the precise role of PRLR in promoting growth of breast cancer and other tumor types remains an area of active investigation (2). Alternate Names delta 4-delta 7/11 truncated prolactin receptor; delta 4-SF1b truncated prolactin receptor; HPRL; hPRL receptor; hPRLrI; MFAB; PRL-R; PRLR; Prolactin receptor; prolactin receptor delta 7/11; RI-PRLR; secreted prolactin binding protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 65 Source/Isotype: Rabbit IgG