CST, 13561T, DDC (D6N8N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DOPA decarboxylase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DDC (D6N8N) Rabbit Monoclonal Antibody (CST #13561) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DDC (D6N8N) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDC protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human DDC protein. Background L-DOPA decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-DOPA to dopamine (1) and L-5HTP to serotonin (2). By catalyzing the reaction to produce dopamine, DDC is involved in many important metabolic processes and plays a central role in the complex neuroendocrine-immune regulatory network (1). DDC is expressed in the central nervous system (3), but has also been detected in some peripheral organs such as the liver and adrenal gland, as well as leukocytes of rat and human (1). DDC is thought to be the sole enzyme responsible for the synthesis of the trace amines 2-phenylethylamine, p-tyramine, and tryptamine, which are considered to act as neuromodulators (2,4). DDC is also regarded as a general biomarker for neuroendocrine tumors (3). Alternate Names AADC; Aromatic-L-amino-acid decarboxylase; DDC; DOPA decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG