CST, 13562S, Kindlin-2 Antibody

Polyclonal Antibody for studying kindlin-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Kindlin-2 Antibody (CST #13562) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Kindlin-2 Antibody recognizes endogenous levels of total kindlin-2 protein. This antibody does not cross-react with kindlin-1 or kindlin-3 proteins. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn502 of human kindlin-2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3). Kindlin-2 regulates integrin β3 mediated adhesion and migration (4,5) and contributes to Wnt signaling through interaction with β-catenin and activation of Wnt target genes (6). Research studies indicate that kindlin-2 expression in pancreatic and gastric cancers may be indicative of poor prognosis (7,8) and that kindlin-2 may support invasiveness in breast cancer (9). Additional studies show that expression of kindlin-2 in prostate cancer cells can protect cells from cisplatin-induced cell death (10). Alternate Names DKFZp686G11125; FERM2; Fermitin family homolog 2; fermitin family homolog 2 (Drosophila); fermitin family member 2; FERMT2; FLJ34213; FLJ44462; KIND2; kindlin 2; Kindlin-2; MIG-2; MIG2; mitogen inducible gene 2 protein; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112; UNC112B Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 77 SOURCE: Rabbit