CST, 13585S, UBLE1A/SAE1 Antibody

Polyclonal Antibody for studying UBLE1A. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, UBLE1A/SAE1 Antibody (CST #13585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBLE1A/SAE1 Antibody recognizes endogenous levels of total UBLE1A/SAE1 protein. This antibody does not cross-react with NAE1 or UBE1 proteins. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys195 of human UBLE1A/SAE1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The process of SUMO conjugation to target proteins is similar to the molecular chain of events observed with ubiquitin (1). SUMO is conjugated to target proteins through the coordinated action of the cellular SUMO conjugation machinery, which consists of the E1, E2, and E3 enzymes (2). The canonical SUMO E1 activating enzyme is a heterodimer consisting of Ubiquitin-like 1-activating enzyme E1A (UBLE1A, SAE1) and UBLE1B (SAE2, UBA2) subunits. Mature SUMO is activated by E1 in an ATP-dependent reaction that generates adenylated SUMO, which functions as a high-energy intermediate in the formation of a thioester linkage between SUMO and Cys173 of SAE2 (3,4). SUMO is subsequently transferred from SAE2 to the SUMO E2 conjugating enzyme UBE2I (5). Research studies indicate that UBLE1A (SAE1) is a nuclear protein and c-Myc transcriptional target whose expression is required for Myc-driven tumorigenesis (6-8). Alternate Names activator of SUMO1; AOS1; FLJ3091; HSPC140; SAE1; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; SUMO-activating enzyme subunit 1, N-terminally processed; SUMO1 activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit