CST, 13608S, Phospho-TTF-1 (Ser327) Antibody

Polyclonal Antibody for studying TTF-1 (Ser327) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-TTF-1 (Ser327) Antibody (CST #13608) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-TTF-1 (Ser327) Antibody recognizes endogenous levels of TTF-1 protein only when phosphorylated at Ser327. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser327 of human TTF-1 protein. Background Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6). Research studies have shown that activation of the Raf/MEK/ERK signaling pathway results in phosphorylation of TTF-1 at multiple residues, including Ser18, Ser327, and Ser337 (7). Concomitant phosphorylation at these residues was shown to suppress the transcriptional activity of TTF-1 in a rat follicular thyroid cell model (7). These findings are consistent with previous studies demonstrating a reduction in thyroid-specific gene expression in Ras-transformed thyroid cells (8,9). Alternate Names BCH; BHC; Homeobox protein NK-2 homolog A; Homeobox protein Nkx-2.1; NK-2; NK-2 homolog A; NK2 homeobox 1; NKX2-1; NKX2.1; NKX21; NKX2A; NMTC1; T/EBP; TEBP; Thyroid nuclear factor 1; Thyroid transcription factor 1; Thyroid-specific enhancer-binding protein; TITF1; TTF-1; TTF1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit