CST, 13627S, IL-1 beta (D3U3E) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying IL1 beta. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-1 beta (D3U3E) Rabbit Monoclonal Antibody (PE Conjugate) (CST #13627) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL-1 beta (D3U3E) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total IL-1β protein and can detect up to 100 pg of recombinant mature IL-1β. This antibody conjugate is not observed to detect endogenous levels of mature IL-1β. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein. Background Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. Alternate Names Catabolin; IL-1; IL-1 beta; IL1-BETA; IL1B; IL1beta; IL1F2; interleukin 1 beta; interleukin 1, beta; interleukin 1beta; Interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG