CST, 13641S, MED26 Antibody

Polyclonal Antibody for studying MED26. Validated for Western Blotting. Highly specific and rigorously validated in-house, MED26 Antibody (CST #13641) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MED26 Antibody recognizes endogenous levels of total MED26 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MED26 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops' that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter and works to sustain proper chromatin architecture during active transcription (6-8). Mediator complex subunit 26 (MED26) plays a critical role in the transition of RNAPII from the initiation to elongation phase of transcription. The amino-terminal domain of MED26 is required for recruitment of the ELL- and pTEFb-containing complexes to target genes, which facilitates transcription elongation (9). Alternate Names Activator-recruited cofactor 70 kDa component; ARC70; Cofactor required for Sp1 transcriptional activation subunit 7; cofactor required for Sp1 transcriptional activation, subunit 7 (70kD); cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa; CRSP complex subunit 7; CRSP7; CRSP70; MED26; Mediator complex subunit 26; Mediator of RNA polymerase II transcription subunit 26; Transcriptional coactivator CRSP70 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit