CST, 13650S, Langerin (D9H7R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD207. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Langerin (D9H7R) Rabbit Monoclonal Antibody (CST #13650) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49830 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Langerin (D9H7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Langerin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human Langerin protein. Background Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin, including Langerhans cells in the epidermis and langerin /CD103 dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8). Alternate Names C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; CD207; CD207 antigen, langerin; CD207 molecule; CD207 molecule, langerin; CLC4K; CLEC4K; Langerhans cell specific c-type lectin; Langerin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG