CST, 13684S, PD-L1 (E1L3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PD-L1. Validated for WB,IP,IHC,IHC,F. Available in 3 sizes. Highly specific and rigorously validated in-house, PD-L1 (E1L3N) Rabbit Monoclonal Antibody (CST #13684) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85164 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PD-L1 (E1L3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total PD-L1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein. Background Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). Alternate Names B7 homolog 1; B7-H; B7-H1; B7H1; CD274; CD274 antigen; CD274 molecule; hPD-L1; MGC142294; MGC142296; PD-L1; PD1L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; Programmed death ligand 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG