CST, 13805S, XAF1 (E1E4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BIRC4BP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, XAF1 (E1E4O) Rabbit Monoclonal Antibody (CST #13805) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity XAF1 (E1E4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total XAF1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val210 of human XAF1 protein. Background X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) is a zinc finger protein that antagonizes the anti-apoptotic activity of XIAP (1,2). XIAP, a member of the inhibitor of apoptosis (IAP) family, inhibits apoptosis by direct inhibition of caspases (3; reviewed in 4). XAF1 is widely expressed in normal tissues, with highest levels in the heart and ovary, but is mostly reduced in cancer lines (1,2). Expression of XAF1 can be induced by interferons via Stat transcriptional activity (5-7). The levels of XAF1 have been shown to be inversely correlated with p53, and p53 is directly responsible for inhibiting XAF1 transcription (8,9). A number of studies have shown that XAF1 can function as a tumor suppressor protein, and decreased levels of XAF1 are found in a variety of different cancers (10-13). Research studies suggest that expression of XAF1 may be a prognostic biomarker for some cancers (14-16). Alternate Names BIRC4 binding protein; BIRC4-binding protein; BIRC4BP; HSXIAPAF1; XAF1; XIAP associated factor 1; XIAP-associated factor 1; XIAPAF1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32, 38 Source/Isotype: Rabbit IgG