CST, 13808S, PTPN14 (D5T6Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTPN14. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PTPN14 (D5T6Y) Rabbit Monoclonal Antibody (CST #13808) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PTPN14 (D5T6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPN14 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu737 of human PTPN14 protein. Background Tyrosine-protein phosphatase non-receptor type-14 (PTPN14, Pez, PTPD2 and PTP36) is an evolutionarily conserved non-membrane tyrosine phosphatase with homology to the band 4.1 family of proteins (1-3). The PTPN14 protein contains an amino-terminal FERM (4.1-ezrin-radixin-moesin) domain, which suggests plasma membrane localization of the protein, and a carboxy-terminal protein tyrosine phosphatase (PTP) domain (4). Research studies have identified possible roles for PTPN14 in multiple, diverse signaling pathways, including cell growth and proliferation, cell migration and adhesion, and development. The PTPN14 phosphatase regulates the subcellular localization of YAP in a cell density-dependent manner, indicating a role for PTPN14 in the Hippo signaling pathway (5). The PTPN14 homolog Pez localizes to adherens junctions, where it may regulate cell motility through dephosphorylation of β-catenin (3). PTPN14 may play a role in epithelial-mesenchymal transition through effects on the TGF-β signaling pathway (6), and interacts with VEGFR3, a receptor tyrosine kinase involved in lymphangiogenesis (7). Loss-of-function mutations in the gene are associated with colorectal cancer (8), and choanal atresia and lymphedema, an autosomal recessive disorder characterized by defects in both nasal passage development and lymphangiogenesis (7). Alternate Names CATLPH; cytoskeletal-associated protein tyrosine phosphatase; MGC126803; PEZ; protein tyrosine phosphatase non-receptor type 14; protein tyrosine phosphatase, non-receptor type 14; Protein-tyrosine phosphatase pez; PTN14; PTP36; PTPD2; PTPN14; Tyrosine-protein phosphatase non-receptor type 14 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG