CST, 13809S, P2X7 Receptor (E1E8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P2X7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, P2X7 Receptor (E1E8T) Rabbit Monoclonal Antibody (CST #13809) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity P2X7 Receptor (E1E8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total P2X7 receptor protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu462 of human P2X7 receptor protein. Background P2X purinergic receptors are ATP-gated ion channels involved in physiological processes that include inflammation, afferent sensory signaling, and sympathetic motor nerve activity. Seven different vertebrate genes ( - ) encode for individual receptor protein subunits (1). All P2X subunit proteins share similar protein domain structure, but can differ in overall protein length from 384 to 595 amino acids. Each P2X subunit is composed of amino- and carboxy-terminal intracellular domains, two transmembrane domains, and a large extracellular loop that contains ten evenly spaced cysteines and multiple glycosylation sites (2). P2X receptors are found in a variety of cell types and tissues, including central and peripheral nervous system neurons and glial cells, autonomic and sensory neurons, bone, muscle, and hematopoietic tissues (1). Purinoceptor 7 (P2X7) is a homotrimer involved in diverse cellular responses, including inflammation mediated by phospholipase A2, phospholipase D, MAP kinase, and NF-κB activation (3,4). Research studies suggest that P2X7 receptors promote apoptosis by regulating release of IL-1β in neurodegenerative disorders associated with inflammation (5). Microglial P2X7 receptors may contribute to neuroinflammatory responses in the ATP-rich site of neuronal injury (6) and mediate inflammatory pain (7, 8). Association studies demonstrate a possible causal link between gene polymorphisms and susceptibility to bipolar affective disorder and major depressive disorder (9,10). Alternate Names ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7 receptor; P2Z receptor; Purinergic receptor; purinergic receptor P2X 7; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; purnergic receptor P2X 7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG